PhytophthoraV1, Main, Exploration, bibRecord, 000512

Extraction of Plant DNA by Microneedle Patch for Rapid Detection of Plant Diseases.

Identifieur interne : 000512 ( Main/Exploration ); précédent : 000511; suivant : 000513

Extraction of Plant DNA by Microneedle Patch for Rapid Detection of Plant Diseases.

Auteurs : Rajesh Paul ; Amanda C. Saville ; Jeana C. Hansel ; Yanqi Ye [États-Unis] ; Carmin Ball ; Alyssa Williams ; Xinyuan Chang [République populaire de Chine] ; Guojun Chen [États-Unis] ; Zhen Gu [États-Unis] ; Jean B. Ristaino ; Qingshan Wei

Source :

ACS nano [ 1936-086X ] ; 2019.

RBID : pubmed:31179687

Descripteurs français

KwdFr :
- ADN fongique (composition chimique), ADN fongique (génétique), Aiguilles (MeSH), Codage à barres de l'ADN pour la taxonomie (instrumentation), Codage à barres de l'ADN pour la taxonomie (méthodes), Feuilles de plante (composition chimique), Feuilles de plante (microbiologie), Lycopersicon esculentum (microbiologie), Maladies des plantes (microbiologie), Métagénomique (instrumentation), Métagénomique (méthodes), Phytophthora (génétique), Phytophthora (pathogénicité).
MESH :
- composition chimique : ADN fongique, Feuilles de plante.
- génétique : ADN fongique, Phytophthora.
- microbiologie : Feuilles de plante, Lycopersicon esculentum, Maladies des plantes.
- méthodes : Codage à barres de l'ADN pour la taxonomie, Métagénomique.
- pathogénicité : Phytophthora.
- instrumentation : Aiguilles, Codage à barres de l'ADN pour la taxonomie, Métagénomique.

English descriptors

KwdEn :
- DNA Barcoding, Taxonomic (instrumentation), DNA Barcoding, Taxonomic (methods), DNA, Fungal (chemistry), DNA, Fungal (genetics), Lycopersicon esculentum (microbiology), Metagenomics (instrumentation), Metagenomics (methods), Needles (MeSH), Phytophthora (genetics), Phytophthora (pathogenicity), Plant Diseases (microbiology), Plant Leaves (chemistry), Plant Leaves (microbiology).
MESH :
- chemical , chemistry : DNA, Fungal.
- chemical , genetics : DNA, Fungal.
- chemistry : Plant Leaves.
- genetics : Phytophthora.
- instrumentation : DNA Barcoding, Taxonomic, Metagenomics.
- methods : DNA Barcoding, Taxonomic, Metagenomics.
- microbiology : Lycopersicon esculentum, Plant Diseases, Plant Leaves.
- pathogenicity : Phytophthora.
- Needles.

Abstract

In-field molecular diagnosis of plant diseases via nucleic acid amplification is currently limited by cumbersome protocols for extracting and isolating pathogenic DNA from plant tissues. To address this challenge, a rapid plant DNA extraction method was developed using a disposable polymeric microneedle (MN) patch. By applying MN patches on plant leaves, amplification-assay-ready DNA can be extracted within a minute from different plant species. MN-extracted DNA was used for direct polymerase chain reaction amplification of plant plastid DNA without purification. Furthermore, using this patch device, extraction of plant pathogen DNA ( Phytophthora infestans) from both laboratory-inoculated and field-infected leaf samples was performed for detection of late blight disease in tomato. MN extraction achieved 100% detection rate of late blight infections for samples after 3 days of inoculation when compared to the conventional gold standard cetyltrimethylammonium bromide (CTAB)-based DNA extraction method and 100% detection rate for all blind field samples tested. This simple, cell-lysis-free, and purification-free DNA extraction method could be a transformative approach to facilitate rapid sample preparation for molecular diagnosis of various plant diseases directly in the field.

DOI: 10.1021/acsnano.9b00193
PubMed: 31179687

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000458
to stream Main, to step Curation: 000458

Le document en format XML

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<term>DNA Barcoding, Taxonomic (methods)</term>
<term>DNA, Fungal (chemistry)</term>
<term>DNA, Fungal (genetics)</term>
<term>Lycopersicon esculentum (microbiology)</term>
<term>Metagenomics (instrumentation)</term>
<term>Metagenomics (methods)</term>
<term>Needles (MeSH)</term>
<term>Phytophthora (genetics)</term>
<term>Phytophthora (pathogenicity)</term>
<term>Plant Diseases (microbiology)</term>
<term>Plant Leaves (chemistry)</term>
<term>Plant Leaves (microbiology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN fongique (composition chimique)</term>
<term>ADN fongique (génétique)</term>
<term>Aiguilles (MeSH)</term>
<term>Codage à barres de l'ADN pour la taxonomie (instrumentation)</term>
<term>Codage à barres de l'ADN pour la taxonomie (méthodes)</term>
<term>Feuilles de plante (composition chimique)</term>
<term>Feuilles de plante (microbiologie)</term>
<term>Lycopersicon esculentum (microbiologie)</term>
<term>Maladies des plantes (microbiologie)</term>
<term>Métagénomique (instrumentation)</term>
<term>Métagénomique (méthodes)</term>
<term>Phytophthora (génétique)</term>
<term>Phytophthora (pathogénicité)</term>
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<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Fungal</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Fungal</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Plant Leaves</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>ADN fongique</term>
<term>Feuilles de plante</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phytophthora</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN fongique</term>
<term>Phytophthora</term>
</keywords>
<keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>DNA Barcoding, Taxonomic</term>
<term>Metagenomics</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>DNA Barcoding, Taxonomic</term>
<term>Metagenomics</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Feuilles de plante</term>
<term>Lycopersicon esculentum</term>
<term>Maladies des plantes</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Lycopersicon esculentum</term>
<term>Plant Diseases</term>
<term>Plant Leaves</term>
</keywords>
<keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Codage à barres de l'ADN pour la taxonomie</term>
<term>Métagénomique</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Phytophthora</term>
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<keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Phytophthora</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Needles</term>
</keywords>
<keywords scheme="MESH" qualifier="instrumentation" xml:lang="fr"><term>Aiguilles</term>
<term>Codage à barres de l'ADN pour la taxonomie</term>
<term>Métagénomique</term>
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<front><div type="abstract" xml:lang="en">In-field molecular diagnosis of plant diseases via nucleic acid amplification is currently limited by cumbersome protocols for extracting and isolating pathogenic DNA from plant tissues. To address this challenge, a rapid plant DNA extraction method was developed using a disposable polymeric microneedle (MN) patch. By applying MN patches on plant leaves, amplification-assay-ready DNA can be extracted within a minute from different plant species. MN-extracted DNA was used for direct polymerase chain reaction amplification of plant plastid DNA without purification. Furthermore, using this patch device, extraction of plant pathogen DNA ( Phytophthora infestans) from both laboratory-inoculated and field-infected leaf samples was performed for detection of late blight disease in tomato. MN extraction achieved 100% detection rate of late blight infections for samples after 3 days of inoculation when compared to the conventional gold standard cetyltrimethylammonium bromide (CTAB)-based DNA extraction method and 100% detection rate for all blind field samples tested. This simple, cell-lysis-free, and purification-free DNA extraction method could be a transformative approach to facilitate rapid sample preparation for molecular diagnosis of various plant diseases directly in the field.</div>
</front>
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<ArticleTitle>Extraction of Plant DNA by Microneedle Patch for Rapid Detection of Plant Diseases.</ArticleTitle>
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<Abstract><AbstractText>In-field molecular diagnosis of plant diseases via nucleic acid amplification is currently limited by cumbersome protocols for extracting and isolating pathogenic DNA from plant tissues. To address this challenge, a rapid plant DNA extraction method was developed using a disposable polymeric microneedle (MN) patch. By applying MN patches on plant leaves, amplification-assay-ready DNA can be extracted within a minute from different plant species. MN-extracted DNA was used for direct polymerase chain reaction amplification of plant plastid DNA without purification. Furthermore, using this patch device, extraction of plant pathogen DNA ( Phytophthora infestans) from both laboratory-inoculated and field-infected leaf samples was performed for detection of late blight disease in tomato. MN extraction achieved 100% detection rate of late blight infections for samples after 3 days of inoculation when compared to the conventional gold standard cetyltrimethylammonium bromide (CTAB)-based DNA extraction method and 100% detection rate for all blind field samples tested. This simple, cell-lysis-free, and purification-free DNA extraction method could be a transformative approach to facilitate rapid sample preparation for molecular diagnosis of various plant diseases directly in the field.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Paul</LastName>
<ForeName>Rajesh</ForeName>
<Initials>R</Initials>
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<Author ValidYN="Y"><LastName>Saville</LastName>
<ForeName>Amanda C</ForeName>
<Initials>AC</Initials>
</Author>
<Author ValidYN="Y"><LastName>Hansel</LastName>
<ForeName>Jeana C</ForeName>
<Initials>JC</Initials>
</Author>
<Author ValidYN="Y"><LastName>Ye</LastName>
<ForeName>Yanqi</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>Joint Department of Biomedical Engineering , University of North Carolina at Chapel Hill, North Carolina State University , Raleigh , North Carolina 27695 , United States.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Ball</LastName>
<ForeName>Carmin</ForeName>
<Initials>C</Initials>
</Author>
<Author ValidYN="Y"><LastName>Williams</LastName>
<ForeName>Alyssa</ForeName>
<Initials>A</Initials>
</Author>
<Author ValidYN="Y"><LastName>Chang</LastName>
<ForeName>Xinyuan</ForeName>
<Initials>X</Initials>
<AffiliationInfo><Affiliation>School of Chemical Engineering and Technology , Tianjin University , Tianjin 300350 , P.R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chen</LastName>
<ForeName>Guojun</ForeName>
<Initials>G</Initials>
<AffiliationInfo><Affiliation>Joint Department of Biomedical Engineering , University of North Carolina at Chapel Hill, North Carolina State University , Raleigh , North Carolina 27695 , United States.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Gu</LastName>
<ForeName>Zhen</ForeName>
<Initials>Z</Initials>
<AffiliationInfo><Affiliation>Joint Department of Biomedical Engineering , University of North Carolina at Chapel Hill, North Carolina State University , Raleigh , North Carolina 27695 , United States.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ristaino</LastName>
<ForeName>Jean B</ForeName>
<Initials>JB</Initials>
</Author>
<Author ValidYN="Y"><LastName>Wei</LastName>
<ForeName>Qingshan</ForeName>
<Initials>Q</Initials>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D058893" MajorTopicYN="N">DNA Barcoding, Taxonomic</DescriptorName>
<QualifierName UI="Q000295" MajorTopicYN="N">instrumentation</QualifierName>
<QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName>
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<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018551" MajorTopicYN="N">Lycopersicon esculentum</DescriptorName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D056186" MajorTopicYN="N">Metagenomics</DescriptorName>
<QualifierName UI="Q000295" MajorTopicYN="N">instrumentation</QualifierName>
<QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009339" MajorTopicYN="N">Needles</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName>
<QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y"></Keyword>
<Keyword MajorTopicYN="Y">DNA extraction</Keyword>
<Keyword MajorTopicYN="Y">microneedle patch</Keyword>
<Keyword MajorTopicYN="Y">nucleic acid amplification</Keyword>
<Keyword MajorTopicYN="Y">plant disease</Keyword>
<Keyword MajorTopicYN="Y">point-of-care diagnostics</Keyword>
</KeywordList>
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<Month>6</Month>
<Day>11</Day>
<Hour>6</Hour>
<Minute>0</Minute>
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<li>États-Unis</li>
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<region><li>Caroline du Nord</li>
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<settlement><li>Chapel Hill (Caroline du Nord)</li>
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<orgName><li>Université de Caroline du Nord à Chapel Hill</li>
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<name sortKey="Paul, Rajesh" sort="Paul, Rajesh" uniqKey="Paul R" first="Rajesh" last="Paul">Rajesh Paul</name>
<name sortKey="Ristaino, Jean B" sort="Ristaino, Jean B" uniqKey="Ristaino J" first="Jean B" last="Ristaino">Jean B. Ristaino</name>
<name sortKey="Saville, Amanda C" sort="Saville, Amanda C" uniqKey="Saville A" first="Amanda C" last="Saville">Amanda C. Saville</name>
<name sortKey="Wei, Qingshan" sort="Wei, Qingshan" uniqKey="Wei Q" first="Qingshan" last="Wei">Qingshan Wei</name>
<name sortKey="Williams, Alyssa" sort="Williams, Alyssa" uniqKey="Williams A" first="Alyssa" last="Williams">Alyssa Williams</name>
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<country name="États-Unis"><region name="Caroline du Nord"><name sortKey="Ye, Yanqi" sort="Ye, Yanqi" uniqKey="Ye Y" first="Yanqi" last="Ye">Yanqi Ye</name>
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<name sortKey="Gu, Zhen" sort="Gu, Zhen" uniqKey="Gu Z" first="Zhen" last="Gu">Zhen Gu</name>
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<country name="République populaire de Chine"><noRegion><name sortKey="Chang, Xinyuan" sort="Chang, Xinyuan" uniqKey="Chang X" first="Xinyuan" last="Chang">Xinyuan Chang</name>
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Extraction of Plant DNA by Microneedle Patch for Rapid Detection of Plant Diseases.

Extraction of Plant DNA by Microneedle Patch for Rapid Detection of Plant Diseases.

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